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目的:观察纳米金(GNP)人耐药肝癌细胞株耐药性的逆转作用。方法:采用氯金酸柠檬酸三钠还原法制备并鉴定;分别用GNP与阿霉素(ADM)组单独或联合作用于ADM耐药的肝癌细胞株Hep G2/ADM,以未处理的Hep G2/ADM细胞为对照,用MTT法检、流式细胞术检测细胞的增殖与凋亡情况;紫外分光光度计检测Hep G2/ADM细胞经ADM单独作用以及GNP与ADM联合作用后细胞内ADM浓度。结果:与对照细胞比较,ADM单独作用及GNP与ADM联合作用后,Hep G2/ADM的增殖均明显抑制、凋亡率明显升高,但后者的作用明显强于前者(均P<0.05),而GNP单独作用对细胞的增殖与凋亡无明显影响(均P>0.05);GNP+ADM作用后,Hep G2/ADM细胞内的ADM含量较ADM单独作用后的ADM含量明显增加[(2.92±0.13)μg/L vs.(1.68±0.74)μg/L,P<0.05]。结论:GNP可增加Hep G2/ADM细胞对ADM的敏感性,该作用可能与其增加Hep G2/ADM细胞内的ADM浓度有关。
Objective: To observe the reversal of drug resistance in human hepatoma cell line GNP. METHODS: The hepatocarcinoma cell line Hep G2 / ADM resistant to ADM was treated with GNP alone or in combination with adriamycin (ADM). The untreated Hep G2 / ADM cells as control, the proliferation and apoptosis of cells were detected by MTT assay and flow cytometry. The ADM concentration in Hep G2 / ADM cells treated with ADM and GNP combined with ADM were detected by UV spectrophotometer. Results: Compared with the control cells, the proliferation of Hep G2 / ADM was significantly inhibited and the apoptosis rate was significantly increased after ADM combined with GNP and ADM, but the effect of the latter was stronger than that of the former (all P <0.05) , While the effect of GNP alone had no significant effect on the proliferation and apoptosis of cells (all P> 0.05). The ADM content in Hep G2 / ADM cells was significantly increased compared with ADM alone ([2.92 ± 0.13) μg / L vs. (1.68 ± 0.74) μg / L, P <0.05]. CONCLUSION: GNP can increase the sensitivity of Hep G2 / ADM cells to ADM, which may be related to the increase of ADM concentration in Hep G2 / ADM cells.