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本文叙述了柚木1—2年生萌条和实生苗茎尖培养方法。取自生长季节和生长停顿季节的柚木茎尖,成苗情况不同。取自生长季节的离体茎尖,培养在附加有BA0.1—1.5毫克/升或BA0.5—1.0毫克/升和IAA0.1—0.5毫克/升的MS培养基上可以增殖成苗。并当培养在温度24°—28℃、光照强度为200D—2500Lux的条件下苗生长健壮。如果将光照强度减至1000Lux,能明显提高增殖率。从培养出的苗上切取一个个的节。每隔一定时间继代培养,所有的节均可发育。在短时期内可以获得大量幼苗。3个月内增殖率可以稳定。据推算一年内一个茎尖外植体可以产生约1.8×10~5小植株。将苗基部在300—700ppM的IBA溶液中处理3—9小时后,培养在无激素的液体MS培养基的泸纸桥上,三周内可全部生根长成正常的完整的小植株。小植株移栽到1∶1的火烧土和表土的盆栽土壤中,成活率不低于75％。自1960年Moyel首次用组织培养获得兰花植株,导致了目前快速繁殖兰花的工业兴起。无性繁殖系的生产,试管品种的商业化是目前植物组织培养应用的主流之一[5]。利用组织培养法进行优良品种和单株的快速繁殖,在多种经济作物及园艺作物上已获得相当大的成功,在一些国家已进入工业生产规模来成批生产“试管植物”[4],近年来具有重要经济价值木本植物的快速繁殖法也有了较快的发展。通过“微繁殖”技术可以解决林木无性繁殖的困难以及用以去除病毒,进行质量、产量和抗病性为目的的早期选择[5][6]。如苹果、柑桔、桉、樱、杨、等[3][5][9][11]。美国已开始实验用“试管植物”苗木植树造林[7]。国际上被誉为重要热带珍贵树种之一的柚木(Tectona grandis L.f.)国外曾进行过组织培养,尚未见有成功的报道。我国引种柚木已有多年历史,它已表现出生长快、林质优的特性。柚木通常以种子繁殖。多年引种栽培表明,在柚木不同种源或同一种源不同个体之间,在生长、抗性方面存在着明显差异。本研究试图为优良种源和单株建立无性系,提供新的技术。
This article describes the 1 - 2 year teak sprouts and seedling shoots culture methods. Taken from the growing season and the growing season of teak shoots, seedlings are different. In vitro shoot tips taken from the growing season can be propagated in MS medium supplemented with BA 0.1-1.5 mg / L or BA 0.5-1.0 mg / L and IAA 0.1-0.5 mg / L. And when cultured at a temperature of 24 ° -28 ℃, the light intensity of 200D-2500Lux conditions under the growth of robust seedlings. If the light intensity reduced to 1000Lux, can significantly improve the proliferation rate. Cut from the nursery shoot one by one section. Subculture at regular intervals, all sections can be developed. In a short period of time can get a lot of seedlings. Proliferation rate can be stable in 3 months. It is estimated that within one year a shoot tip explants can produce about 1.8 × 10 5 plantlets. The basal part of the seedlings was treated in 300-700 pM IBA solution for 3-9 hours and then cultured on the paper-lined bridge of hormone-free liquid MS medium. All of the rooted plants were allowed to grow into normal intact plants within three weeks. Planting small plants transplanted to 1: 1 fire potatoes and topsoil potted soil, the survival rate of not less than 75%. The orchid plant was first obtained from tissue culture in 1960 by Moyel, leading to the industrial boom of the current rapid propagation of orchids. The production of asexual propagation lines and the commercialization of test tube varieties are one of the mainstream applications of plant tissue culture [5]. Tissue culture methods for the rapid propagation of fine varieties and single plants have enjoyed considerable success on a wide range of cash crops and horticultural crops, and in some countries have entered the scale of industrial production for the mass production of “test tube plants” [4] In recent years, the important economic value of rapid reproduction of woody plants has also been rapid development. The “micropropagation” technique can solve the difficulties of clonal propagation of trees and the early options for virus removal, quality, yield and disease resistance [5] [6]. Such as apples, oranges, eucalyptus, cherry, Yang, etc. [3] [5] [9] [11]. The United States has begun experimenting with “in vitro plant” seedlings of afforestation [7]. Tectona grandis L.f., which is internationally recognized as one of the most important tropical precious tree species, has not been reported successfully yet. Teak introduced in China for many years, it has shown rapid growth, excellent quality of the forest. Teak usually breeds seeds. Years of introduction and cultivation showed that there are significant differences in growth and resistance between different provenances of Teak or between individuals of the same source. This study attempts to establish clones for superior provenances and individuals and to provide new techniques.