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目的:观察外源性磷酸肌酸对一次性力竭运动小鼠的影响,探讨其抗疲劳的作用机制。方法:采用小鼠负重游泳建立力竭运动小鼠模型。将小鼠分为力竭给药组(A组)、力竭不给药组(B组)、游泳8min不给药组(C组)、游泳8min给药组(D组)。实验前20min,A、D组小鼠按1000mg/Kg体质量腹腔注射磷酸肌酸钠,B、C组腹腔注射同等剂量的生理盐水。力竭游泳实验按每只小鼠体质量的6%负重进行。用生化比色法检测血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并记录小鼠的力竭时间。结果:A、C、D组的SOD活性较B组显著升高(P<0.05,0.01),D组较A组明显升高(P<0.05)。B组的MDA含量较C组明显升高(P<0.01),A组较D组明显升高(P<0.05)。B组游泳至力竭的时间明显短于A组(P<0.05)。结论:外源性磷酸肌酸增强机体抗疲劳的机制可能与其增强SOD活性、降低MDA含量有关,通过增强自由基的清除来提高机体的抗疲劳能力。
Objective: To observe the effect of exogenous creatine phosphate on exhaustive exercise mice and to explore its anti-fatigue mechanism. Methods: The mouse model of exhausted exercise was established by swimming with mice. The mice were divided into the exhausted group (group A), the exhausted group (group B), the group without swimming for 8 minutes (group C) and the group with swimming for 8 minutes (group D). At 20 minutes before the experiment, mice in groups A and D were injected intraperitoneally with 1000 mg / kg body weight of sodium creatine phosphate. Groups B and C were injected with the same dose of normal saline. Exhaustive swimming experiments were performed at 6% body weight of each mouse. Serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were detected by biochemical colorimetry, and the exhaustion time of mice was recorded. Results: The SOD activity in groups A, C and D was significantly higher than that in group B (P <0.05, 0.01), and that in group D was significantly higher than that in group A (P <0.05). The content of MDA in group B was significantly higher than that in group C (P <0.01), and that in group A was significantly higher than that in group D (P <0.05). The time from swimming to exhaustion in group B was significantly shorter than that in group A (P <0.05). Conclusion: The mechanism of exogenous creatine phosphorylase enhancing the anti-fatigue of the body may be related to its activity of increasing SOD and decreasing the content of MDA. It can improve the anti-fatigue ability of the body by enhancing the clearance of free radicals.