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目的 定量观察治疗量环孢素A(CsA)对大鼠肝脏超微结构的影响。方法 SD大鼠2 4只 ,分为 3组 ,每组 8只。 (1)对照组 :口服橄榄油。 (2 )小剂量CsA组 :口服CsA 2 5mg·kg-1·d-1。(3)大剂量CsA组 :口服CsA 5 0mg·kg-1·d-1。用药 2周后取肝组织制备超薄切片 ,电镜摄片。立体定量分析指标 :(1)体积密度 (Vv)。 (2 )面积密度 (Sv)。 (3)数密度 (Nv)。 (4 )比表面 (δ)。结果 CsA使肝细胞内质网Vv、Sv增加 ,δ值降低 ;微体Vv、Nv降低 ;高尔基体Vv降低 ;糖原减少 ;脂滴Vv增加。CsA对线粒体、溶酶体各项测定值无明显影响。结论 治疗量CsA可引起大鼠肝脏超微结构改变。
Objective To quantitatively observe the therapeutic effect of cyclosporin A (CsA) on the liver ultrastructure in rats. Methods Twenty-four Sprague-Dawley rats were divided into 3 groups with 8 rats in each group. (1) control group: oral olive oil. (2) low-dose CsA group: oral CsA 2 5mg · kg-1 · d-1. (3) high-dose CsA group: oral CsA50mg · kg-1 · d-1. After 2 weeks, the liver tissue was taken to prepare ultrathin section and electron microscope photograph. Stereometric analysis of indicators: (1) bulk density (Vv). (2) Area density (Sv). (3) Number density (Nv). (4) Specific surface (δ). Results CsA increased the content of Vv and Sv in the endoplasmic reticulum, reduced the δ value, reduced the Vv and Nv in the microvessels, decreased the Vv in the Golgi apparatus, decreased the glycogen, and increased the Vv of lipid droplets. CsA had no significant effect on the mitochondria and lysosomes. Conclusion The treatment of CsA can cause ultrastructural changes in rat liver.