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目的 :建立用高效液相色谱法测定血浆中克林沙星浓度的方法。方法 :血浆样品在酸性条件下 ,以Oasis小柱提取环丙沙星为内标 ,采用LichrosorbC18(5 μm)柱 ,流动相为乙腈 - 0 0 5mol·L-1柠檬酸三乙胺溶液 (pH 2 5 ) (2 0∶80 ) ,流速为 1 0mL·min-1,检测波长 30 0nm ,克林沙星和内标的保留时间分别为 7 1min和 4 5min。结果 :线性范围在 0 0 3～10 μg·mL-1(r=0 9998) ,最低检测浓度为 0 0 2 μg·mL-1,RSD <7%。用本法测定了 6只大鼠灌胃剂量 5 0mg·kg-1克林沙星后血浆中克林沙星的浓度经时变化过程。结论 :本方法可用于克林沙星的血药浓度测定及药代动力学研究。
Objective: To establish a method for the determination of clinifloxacin in plasma by high performance liquid chromatography. Methods: The plasma sample was extracted with Oasis cartridge under acidic conditions. The column was on Lichrosorb C18 (5 μm) with a mobile phase of acetonitrile-0.05 mol·L-1 triethylamine citrate solution (20:80), the flow rate was 10 mL · min-1, the detection wavelength was 30 0 nm, and the retention time of clinifloxacin and internal standard was 71 min and 45 min, respectively. Results: The linear range was 0 0 3 ~ 10 μg · mL-1 (r = 0 9998). The lowest detection limit was 0 0 2 μg · mL-1 with RSD <7%. This method was used to determine the time-dependent changes of plasma concentrations of clinifloxacin after 6 ganaxadin doses of 50 mg · kg-1 in rats. Conclusion: This method can be used for the determination of clonifloxacin blood concentration and pharmacokinetic studies.