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为了探讨雌激素促纤溶、抗凝血的心血管保护作用及其细胞和分子生物学机制，应用全自动血凝／纤溶分析仪和发色底物显色测定、组织和细胞原位杂交及激光共聚焦扫描显微镜等技术，观察去卵巢大鼠纤维蛋白原、组织型纤溶酶原激活物和纤溶酶原激活物抑制剂－１活性及基因表达，以及补充雌激素的影响。同时观察人脐静脉内皮细胞中雌激素对组织型纤溶酶原激活物和纤溶酶原激活物抑制剂－１基因表达和活性的影响以及单个内皮细胞胞内游离钙离子的变化。结果发现，去卵巢大鼠血浆纤维蛋白原含量和纤溶酶原激活物抑制剂－１活性高于对照组（Ｐ＜０．０５，Ｐ＜０．０１），血浆组织型纤溶酶原激活物活性低于对照组，补充雌激素后，血浆纤维蛋白原含量和组织型纤溶酶原激活物活性接近对照组。去卵巢大鼠冠状动脉及心肌组织中纤溶酶原激活物抑制剂－１ｍＲＮＡ明显增高。还发现，当雌激素浓度为１０－８和１０－６ｍｏｌ／Ｌ时内皮细胞培养液中组织型纤溶酶原激活物活性高于对照组（Ｐ＜０．０５）；内皮细胞中组织型纤溶酶原激活物ｍＲＮＡ水平随雌激素浓度而升高；而纤溶酶原激活物抑制剂－１ｍＲＮＡ水平在各组中无统计学差异；内皮细胞胞内游离钙离子浓度在雌激素浓度为１０－８ｍｏｌ／Ｌ时降低，１０－６ｍ?
In order to explore the estrogen-induced fibrinolytic and anticoagulant cardiovascular protection and its cellular and molecular biological mechanisms, using automatic hemagglutination / fibrinolysis analyzer and chromogenic substrate assay, tissue and cell in situ hybridization And laser confocal scanning microscopy and other techniques to observe the ovariectomized rats fibrinogen, tissue-type plasminogen activator and plasminogen activator inhibitor-1 activity and gene expression, and the effect of estrogen replacement. The effects of estrogen on the expression and activity of tissue plasminogen activator and plasminogen activator inhibitor-1 in human umbilical vein endothelial cells and the changes of intracellular free calcium in single endothelial cells were also observed. The results showed that plasma fibrinogen content and plasminogen activator inhibitor-1 activity in ovariectomized rats were significantly higher than those in the control group (P <0.05, P <0.01), plasma tissue plasminogen activator Compared with the control group, the activity of plasma fibrinogen and tissue plasminogen activator were similar to those of the control group. In ovariectomized rats, the expression of plasminogen activator inhibitor-1 mRNA in coronary artery and myocardial tissue was significantly increased. Also found that when the estrogen concentration of 10-8 and 10-6mol / L endothelial cell culture medium tissue plasminogen activator activity was higher than the control group (P <0.05); endothelial cells in tissue type fiber The level of mRNA of plasminogen activator increased with estrogen concentration; while the level of plasminogen activator inhibitor-1 mRNA had no significant difference in all groups; the intracellular free calcium concentration of endothelial cells was higher than that of estrogen -8mol / L when reduced, 10-6m?