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取１例湖南丁型肝炎病毒（ＨＤＶ）ＲＮＡ阳性血清，经强变性剂法抽提ＨＤＶＲＮＡ，逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）扩增，扩增的ＨＤＶＣＤＮＡ片段重组到ｐＵＣ１８质粒中，获得了中国湖南株ＨＤＶＣＤＮＡ（４３３－８７０）片段克隆，ＤＮＡ测序结果显示，该片段（４３８ｈｐ长，包括一端ＰＣＲ引物２５ｈｐ）与已知的美国、意大利、法国、诺鲁和中国台湾５株ＨＤＶｃＤＮＡ相同片段比较，同源性分别为９１．３％，９４．５％，９１．３％，８４．５％和８９．３％，其中与ＨＤＶＲＮＡ复制密切相关的第一个高度保守区与美国株、意大利株和法国株完全同源。
HDV RNA positive sera from Hunan hepatitis B virus (HDV) were obtained. HDV RNA was extracted by the strong denaturant method and amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). The amplified HDV cDNA fragment was recombined into pUC18 plasmid, The fragment of HDVCDNA (433-870) was obtained from Chinese Hunan strain. DNA sequencing showed that this fragment (438 hp long including 25hp PCR primer) was the same as the known 5 HDV cDNAs from USA, Italy, France, Noro and Taiwan The homology was 91.3%, 94.5%, 91.3%, 84.5% and 89.3%, respectively. Among them, the first highly conserved region closely related to HDV RNA replication was associated with the U.S. strain, Italy and France strains completely homologous.