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【目的】寻找与茄子皮色或果皮花青素合成相关基因连锁的分子标记,检验混合品系法(bulked lines analysis)在筛选分子标记中的应用。【方法】采用AFLP技术,以具有不同果皮颜色的茄子及近缘种为材料,采用单株检测和混合品系法分别筛选与茄子果皮颜色相关基因连锁的分子标记。【结果】对58份高代自交系材料进行单株检测(亲缘关系分析),筛选到了1个与茄子紫红、紫黑果色相关的共显性标记。测序结果表明,片段长度分别为107和108bp,为发生插入/缺失突变的同源序列;将其转化为SCAR标记,对136份茄子材料进行验证,在其中111份紫红、紫黑皮色材料中,相关符合率为90%,在白色及绿色材料中表现为不规律分布。最后以混合品系法,在两池内进一步筛选到了6个多态性标记,对6个标记进行单株验证,确定了它们与紫红、紫黑果色相关。【结论】本试验所获得的SCAR标记、AFLP标记可以用于茄子果色分子标记辅助育种。
【Objective】 The objective of this study was to find molecular markers linked to the genes related to anthocyanin synthesis in egg skin or pericarp and to test the application of bulked lines analysis in screening molecular markers. 【Method】 The AFLP technique was used to screen the molecular markers linked to the color-related genes of eggplant peel with eggplant and its relatives with different peel color. 【Result】 Fifty-eight high-generation inbred lines were tested for single plant (phylogenetic analysis), and a co-dominant marker was screened for the purple and purple color of eggplant. Sequencing results showed that the fragments were 107 and 108 bp in length, respectively, which were homologous sequences of insertion / deletion mutation. The SCAR marker was used to identify 136 eggplant materials. Among 111 mauve and purple black skin materials, , The relevant coincidence rate was 90%, showing irregular distribution in white and green materials. At last, six polymorphic markers were further screened in the two pools by the hybrid method, and the six markers were tested individually to confirm their correlation with mauve and purple-black color. 【Conclusion】 The SCAR and AFLP markers obtained in this experiment can be used for fruit color molecular marker-assisted breeding of eggplant.