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目的建立同时测定夏枯草Prunella vulgaris中迷迭香酸、异迷迭香酸苷、咖啡酸、芦丁、木犀草素5种活性成分的方法,并对不同产地野生与栽培夏枯草进行测定研究,比较夏枯草野生品与栽培品在酚酸和黄酮类成分量上的差异。方法采用Agilent 5HC-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸水溶液(B)为流动相;柱温30℃;体积流量1.0 mL/min;检测波长280 nm。以独立样本t检验,辅以聚类分析和相关性分析方法进行评价。结果独立样本t检验表明,夏枯草野生品与栽培品在迷迭香酸、芦丁、咖啡酸和木犀草素4种成分量上差异显著(P<0.01),而异迷迭香酸苷量无差异(P>0.05)。聚类分析结果显示,大部分产地野生与栽培夏枯草能被正确区分。相关性分析结果表明,果穗长度与夏枯草主要成分量无明显关系。结论所建立的测定方法简单、可行,可以作为夏枯草品质评价方法之一。
OBJECTIVE To establish a method for the simultaneous determination of five active ingredients of rosmarinic acid, isomerises, caffeic acid, rutin and luteolin in Prunella vulgaris, and to determine the contents of wild and cultivated Prunella vulgaris in different producing areas. The differences of the contents of phenolic acids and flavonoids between wild and cultivated Prunella vulgaris were compared. Methods The mobile phase consisted of acetonitrile (A) - 0.1% phosphoric acid solution (B) with a mobile phase of 5HC-C 18 (250 mm × 4.6 mm, 5 μm) at a column temperature of 30 ℃ and a volume flow rate of 1.0 mL / Detection wavelength 280 nm. Independent samples t test, supplemented by cluster analysis and correlation analysis method for evaluation. Results The independent sample t test showed that the wild and cultivated products of Prunella vulgaris had significant differences (P <0.01) in four components of rosmarinic acid, rutin, caffeic acid and luteolin, while the content of isradamacin No difference (P> 0.05). Cluster analysis showed that the majority of wild and cultivated Prunella can be correctly distinguished. Correlation analysis showed that there was no significant correlation between the ear length and the main components of Prunella vulgaris. Conclusion The established assay is simple and feasible and can be used as one of the methods for the quality evaluation of Prunella vulgaris.