采用ＤＮＡ指纹分析和聚丙烯酰胺凝胶电泳法，对一例与公驴交配生育了后代的母后代进行了亲缘鉴定和血清蛋白、酯酶遗传的分析。结果可以认定其亲子关系并证实母骡生育的事实。虽然本例母的后代在Ｐｒ、Ａｌ、Ｐａ和Ｈｂ、Ｅｓ等基因座位上的基因表达倾向于驴，但其外貌仍明显地带有种间杂种的特征。因此，尚不能简单地认为其已“回归”为纯种的驴。 DNA fingerprinting and polyacrylamide gel electrophoresis were used to analyze the genetic relationship and serum protein and esterase genetic analysis of a progeny of the maternal offspring mating with the male donkey. The result can be identified as a parent-child relationship and confirm the fact that female mules are born. Although the progenies in this case have a predilection for donkey expression at loci such as Pr, Al, Pa and Hb, Es, their appearance is clearly marked by interspecific hybridization. Therefore, it can not yet be simply assumed that it has “returned” to a purebred donkey.