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神经管缺陷(NTD)胎儿的产前诊断,主要是在妊娠16～17周检测羊水中甲胎蛋白(AFP)水平和用凝胶电泳分析羊水中的乙酰胆碱酯酶。最近 Crandall 等(1987)提出了在孕早期检测羊水中 AFP 水平作为对 NTD 产前诊断的方法,但必须建立孕15周前的 AFP 正常对照值。为避免定量分析的误差,作者利用凝胶电泳对羊水乙酰胆碱酯酶(AChE)进行定量分析。采用两种方法抽取羊水:①正常孕妇终止妊娠时抽取羊水4～6ml;②对具有21-羟化酶缺陷胎儿的孕妇早期经腹穿刺抽取羊水1～1.5ml。抽取羊水时避免母血污染。共分析121个样品,孕9周前全部羊水的 AChE 电
Prenatal diagnosis of neural tube defects (NTD) in fetuses, mainly in the detection of amniotic fluid AFP (AFP) levels 16 to 17 weeks of gestation and analysis of amniotic fluid acetylcholinesterase by gel electrophoresis. Recently, Crandall et al. (1987) proposed the detection of AFP levels in amniotic fluid during early pregnancy as a method of prenatal diagnosis of NTD. However, normal AFP levels must be established 15 weeks prior to pregnancy. To avoid quantitative analysis of the error, the authors used gel electrophoresis of amniotic fluid acetylcholinesterase (AChE) quantitative analysis. Amniotic fluid extraction using two methods: ① normal pregnant women to withdraw amniotic fluid during termination of pregnancy 4 ~ 6ml; ② pregnant women with 21-hydroxylase-deficient fetus by abdominal puncture early amniotic fluid extraction 1 ~ 1.5ml. Avoid maternal blood contamination when drawing amniotic fluid. A total of 121 samples were analyzed for AChE in all amniotic fluid nine weeks before pregnancy