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采用大鼠、小鼠脑缺血性缺氧以及结扎小鼠双侧颈总动脉造成的脑缺血再灌注损伤两种模型观察童智灵口服液对动物中枢神经系统的影响。并测定大鼠全血ＬＰＯ、ＳＯＤ和ＧＳＨＰｘ含量，以探讨其作用机制。研究结果表明，两种剂量灌胃给药，均可显著延长大、小鼠脑缺血性缺氧后的存活时间，并减少小鼠脑缺血４５ｍｉｎ再灌注６ｈ的卒中指数和２４ｈ内缺血再灌注损伤引起的死亡率。ｉｇ给药１５天，可降低大鼠全血ＬＰＯ含量，提高ＳＯＤ、ＧＳＨＰｘ活性，产生明显的抗氧化作用。说明，童智灵口服液可提高大、小鼠对脑缺氧的耐受能力并可对抗小鼠脑缺血及缺血再灌注损伤，对脑缺血小鼠具有保护作用。其作用机制可能与提高动物体内氧化酶活性，抑制自由基生成有关。
The effects of Tongzhiling oral liquid on the central nervous system in animals were observed with rat and mouse cerebral ischemic hypoxia and cerebral ischemic reperfusion injury caused by bilateral common carotid artery ligation. The contents of LPO, SOD and GSH-Px in rat whole blood were measured to explore the mechanism. The results showed that two doses of gavage can significantly prolong the survival time of cerebral ischemic hypoxia in rats and mice, and reduce the stroke index and intracerebral ischemia within 24 hours after cerebral ischemia at 45 min and reperfusion for 6 h. Mortality caused by reperfusion injury. After 15 days of ig administration, the content of LPO in the whole blood of rats could be decreased, SOD, GSHPx activity was increased, and obvious antioxidative effects were produced. It shows that Tongzhiling oral liquid can improve the tolerance of large and mice to cerebral hypoxia and can protect against cerebral ischemia and ischemia-reperfusion injury in mice, and has a protective effect on cerebral ischemia mice. Its mechanism of action may be related to the increase of oxidase activity in animals and inhibition of free radical production.