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研究了钴与二溴羟基苯基荧光酮在溴化十六烷基吡啶—乳化剂ＯＰ复配型微乳液介质中的显色反应，钴与ＤＢＨ—ＰＦ在ｐＨ１０．５的硼砂—氢氧化钠缓冲溶液中，形成１３的稳定配合物．配合物的最大吸收波长为６４０ｎｍ，表观摩尔吸光系数为１．９×１０５Ｌｍｏｌ－１ｃｍ－１，钴量在０～６ｍｇ／１０Ｌ范围内符合比尔定律．本法采用巯基葡聚糖凝胶分离共存离子富集钴，提高了方法的选择性，使方法的检测限降低．该法用于维生素Ｂ１２样品的测定，结果准确可靠．
The color reaction of cobalt with dibromohydroxyphenylfluorone in cetyl pyridinium bromide emulsifier OP complex microemulsion medium was studied. The reaction of cobalt with DBH-PF in borax-sodium hydroxide at pH 10.5 Buffer solution to form a stable complex of 13. The maximum absorption wavelength of the complex is 640nm, the apparent molar absorptivity is 1.9 × 105Lmol-1cm-1 and the Beer’s law is obeyed in the range of 0-6mg / 10L of cobalt. This method uses mercapto-dextran gel separation of coexisting ions to enrich cobalt, increasing the selectivity of the method and reducing the detection limit of the method. The method is used for the determination of vitamin B12 samples, the results are accurate and reliable.