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了解术前化疗对神经母细胞瘤增殖活性的影响,并探讨其作用机理。应用图象细胞仪检测术前化疗与一期手术组神经母细胞瘤的DNA含量和细胞时相分布百分比并作差异分析。10例一期手术组近异倍体2例,异倍体8例,DI=1.84±0.69;术前化疗组二倍体7例,近异倍体1例,DI=1.05±0.1,差异显著(P<0.01)。术前化疗组G0+G1时相比例升高,S和G2+M时相比例降低。术前化疗具有促使神经母细胞瘤细胞增殖活性降低的积极作用。
To understand the impact of preoperative chemotherapy on the proliferation of neuroblastoma activity, and explore its mechanism of action. Using image cytometry to detect preoperative chemotherapy and surgery group neuroblastoma DNA content and the percentage of cell phase distribution and make a difference analysis. In the 10 cases of primary surgery group, there were 2 aneuploidy and 8 aneuploidy, DI = 1.84 ± 0.69. There were 7 cases of diploid and 1 case of nearly aneuploid in preoperation group. DI = 1. 05 ± 0.1, the difference was significant (P <0.01). Preoperative chemotherapy group G0 + G1 proportion increased, S and G2 + M proportion decreased. Preoperative chemotherapy has the positive effect of reducing the proliferation activity of neuroblastoma cells.