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目的对荆豆凝集素修饰牛血清白蛋白脂质体的黏附性进行评价。方法采用逆向蒸发法制备了荧光标记的普通脂质体和荆豆凝集素修饰的脂质体,通过小鼠胃肠道组织和荧光标记脂质体的离体孵化法考察了制剂的体外黏附性;通过制剂灌胃后的在体实验法考察了其体内黏附性。结果荆豆凝集素修饰的脂质体在小鼠胃、不含派伊尔氏结(peyer's patches,PPs)小肠和结肠中的黏附量与普通脂质体组相比没有明显差异(P>0.05),但在有PPs小肠区域,黏附脂质体量显著增加(P<0.05),且黏附时间可达24 h以上。结论荆豆凝集素修饰的脂质体可以长时间特异性黏附于小鼠含有PPs的小肠部位,从而增强口服黏膜免疫的靶向性和长效性。
Objective To evaluate the adhesion of lectin modified bovine serum albumin liposomes. Methods Fluorescent labeled liposomes and liposomes modified by sterilized lentil were prepared by reverse evaporation method. The in vitro adhesion of the preparations was evaluated by in vitro incubation of mouse gastrointestinal tissues and fluorescent labeled liposomes ; In vivo test by intragastric administration after formulation gavage. Results There was no significant difference in the adhesion between the liposomes modified by Vitex peel agglutinin and the small intestine and colon without peyer's patches (PPs) compared with the normal liposome group (P> 0.05 ), But there was a significant increase in the amount of liposomes in the small intestine with PPs (P <0.05), and the adhesion time was up to 24 h. Conclusion Vitellogenin-modified liposomes can adhere specifically to the small intestine of mice containing PPs for a long time, so as to enhance the targeting and long-term efficacy of oral mucosal immunity.