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一个有效的有固定分子氮能力的共生关系的产生,包括着豆科寄主和固氮菌共生体之间许多特殊的相互作用。环境的改变,寄主或固氮菌基因组的改变能产生无效的共生关系。无效根瘤和有效根瘤在生理和结构上均可不同,无效根瘤也许可能在结构上与有效根瘤似乎是相似的但却不固氮。无效性是观察固氮作用中重要步骤的有用工具,已经发表了许多豆科植物无效根瘤的遗传,生理和结构的研究。只有少数几个研究是关于苜蓿的,所介绍的仅限于根瘤菌所决定的无效性。在我们的固氮研究过程中,发现了苜蓿MnpL-480,其根瘤显然没有固定分子氮的能力。与种植在无硝酸盐的温室沙床上的正常植株相比,MnpL-480很矮小而且缺绿。
An effective symbiotic relationship with the ability of fixed molecules to produce nitrogen involves many specific interactions between legume host and nitrogen-fixing colonies. Changes in the environment, host or Azotobacter genome changes can produce inefficient symbiotic relationship. Invalid nodules and effective nodules can both be physiologically and structurally distinct, and ineffective nodules may likely appear to be structurally similar to effective nodules but not to nitrogen fixation. Invalidity is a useful tool for the observation of important steps in nitrogen fixation and many genetic, physiological and structural studies have been published on invalid nodules of leguminous plants. Only a few studies have been conducted on alfalfa, and the introduction is limited to the ineffectiveness of rhizobia. In our nitrogen fixation study, alfalfa MnpL-480 was found, whose root nodules apparently lacked the ability to fix molecular nitrogen. MnpL-480 is short and greenish compared to normal plants grown on nitrate-free greenhouse sand beds.