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目的根据吖啶橙和罗丹明6 G两分子间能够有效地发生荧光共振能量转移,建立一种检测铅离子的新方法。方法在表面活性剂SDS的存在下,吖啶橙和罗丹明6 G分子间发生荧光共振能量转移,使罗丹明6 G的荧光强度增大;加入铅离子后能对AO-R6 G体系的荧光产生猝灭作用,其荧光猝灭程度随着铅离子浓度的增大而增强,据此建立了检测Pb2+的新方法。结果当Pb2+浓度为2.0×10-7mol/L~3.0×10-6mol/L时,与荧光猝灭程度ΔF有良好的线性关系,线性回归方程为y=16.34x+42.79,相关系数(r)=0.998,检出限为6.06×10-8mol/L,相对标准偏差(RSD)为3.4%~5.3%,加标回收率为95.1%~96.5%。结论本方法操作简便、灵敏,可用于实际水样中铅含量的测定。
OBJECTIVE To establish a new method for the determination of lead ions based on the fluorescence resonance energy transfer between acridine orange and rhodamine 6G molecules. Methods Fluorescence resonance energy transfer between acridine orange and rhodamine 6 G was carried out in the presence of surfactant SDS to increase the fluorescence intensity of rhodamine 6 G. After the addition of lead ions, the fluorescence of AO-R6 G system Quenching effect, the degree of fluorescence quenching increases with the lead concentration increases, thus establishing a new method for the detection of Pb2 +. Results When Pb2 + concentration was 2.0 × 10-7mol / L to 3.0 × 10-6mol / L, there was a good linear relationship with the fluorescence quenching degree ΔF. The linear regression equation was y = 16.34x + 42.79, and the correlation coefficient (r) = 0.998, the detection limit was 6.06 × 10-8mol / L, the relative standard deviation (RSD) was 3.4% -5.3%, and the recoveries were 95.1% -96.5%. Conclusion The method is simple, sensitive and can be used for the determination of lead in water samples.