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目的:对持续喘息患儿不同呼吸道病毒进行检测分析其检出率及临床特点。方法:对2009年至2010年在我院住院的61例持续喘息住院患儿应用逆转录PCR(RT-PCR)方法检测人类偏肺病毒(hMPV),实时PCR检测人类博卡病毒(hBoV),同时采用直接免疫荧光法检测呼吸道分泌物中呼吸道合胞病毒(RSV)、流感病毒(A、B型)、副流感病毒(PinfⅠ～Ⅲ)和腺病毒(ADV)。结果:(1)61例标本中病毒检测阳性27例,总检出率44.3%,其中单一病毒感染20例,分别为hMPV 10例(16.4%)、hBoV 7例(11.5%)、RSV 2例(3.3%)、Pinf-Ⅲ1例(1.6%);两种病毒感染7例,分别为hMPV+hBoV 4例(6.6%)、hBoV+PinfⅢ2例(3.3%)、RSV+hBoV 1例(1.6%)。(2)hMPV和hBoV感染的标本主要来自于冬春季。(3)hMPV与hBoV感染患儿的发病年龄相近;hMPV与hBoV感染引起的临床表现如发热、咳嗽、喘息和呼吸困难等比较差异均无统计学意义(P>0.05)。结论:hMPV和hBoV与儿童持续喘息有一定相关性,检出高峰在冬春季,两者感染后的临床症状相似。
OBJECTIVE: To detect and analyze the different respiratory viruses in children with persistent wheezing and analyze the detection rate and clinical features. Methods: Sixty-one children with persistent wheezing and hospitalized in our hospital from 2009 to 2010 were tested for hMPV by real-time polymerase chain reaction (RT-PCR) and hBoV by real-time PCR. The respiratory syncytial virus (RSV), influenza virus (A and B), parainfluenza virus (PinfⅠ-Ⅲ) and adenovirus (ADV) were detected by direct immunofluorescence assay. Results: (1) Twenty-seven cases were detected positive in the 61 samples, with a total detection rate of 44.3%. Among them, 20 cases were infected with a single virus, including hMPV in 10 cases (16.4%), hBoV in 7 cases (3.3%) and 1 case (1.6%) of Pinf-Ⅲ. Seven cases were infected with both viruses, including hMPV + hBoV in 4 cases (6.6%), hBoV + PinfⅢ in 2 cases ). (2) The samples infected with hMPV and hBoV mainly came from winter and spring. (3) The age of onset of hMPV infection was similar to that of hBoV infection. The clinical manifestations of hMPV and hBoV infection were not statistically significant (P> 0.05), such as fever, cough, wheezing and dyspnea. Conclusion: hMPV and hBoV have a certain correlation with persistent wheezing in children. The detection peak is similar in both winter and spring.