【摘 要】
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Objective:To study virulence and regulatory genes (hlyA,ctxB,tcpI) in clinical strains ofVibrio cholerae (V. cholerae), simultaneously.Methods:Three important g
【机 构】
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Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences,Tehran, Iran“,”Dep
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Objective:To study virulence and regulatory genes (hlyA,ctxB,tcpI) in clinical strains ofVibrio cholerae (V. cholerae), simultaneously.Methods:Three important genes,tcpI,hlyA andctxB were used for detection of toxigenic and pathogenicV. cholera by chain reaction assay method. Results:According to the results of thePCR, the incidence ofhlyA,tcpI, andctxB genes in clinical isolates was obtained as94.7% (72 sample),90.8% (69 sample), and92.1% (70 sample), respectively.Five strains possessed all genes exceptctxB, six strains possessed all genes except tcpI, four strains possessed all genes excepthlyA, one strain possessed onlyhlyA and60 strains contained a combination of three genes,IncludinghlyA,ctxB andtcpI.Conclusions:Result show that this method could be reliable to detect toxigenic-pathogenic strains ofV. cholerae in Iran.
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