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[目的]对微波联合大孔树脂提取腊梅花总黄酮的新工艺进行研究。[方法]在不同的提取工艺条件下进行平行试验、正交试验和对比试验。[结果]原料0.500 g的腊梅花粉末,以70%的乙醇溶液作提取剂,提取剂的用量为1∶60(g/ml),微波功率为288 W,在提取时间为450 s下提取次数3为最佳工艺。确定AB-8为腊梅花总黄酮的吸附树脂。其最佳静态吸附工艺条件为:温度30℃,吸附时间3 h;最佳动态解吸工艺条件为:流速2.6 ml/min,上样液pH值4.3,30 ml 70%乙醇洗脱。与溶剂浸提法相比,采用微波法提取腊梅花总黄酮提取时间由20 h缩短为450 s,提取率从79.86%提高到92.23%,而且AB-8分离腊梅花总黄酮分离效果明显,成本低。[结论]微波联合大孔树脂提取腊梅花总黄酮可工业化推广。
[Objective] The research aimed to study the new technology of microwave extraction of total flavonoids from Plum blossom with macroporous resin. [Method] The parallel experiment, the orthogonal experiment and the comparative experiment were carried out under different extraction technological conditions. [Result] The raw material of 0.500 g of camellia flower powder was extracted with 70% ethanol solution, the amount of extractant was 1:60 (g / ml), the microwave power was 288 W and the extraction time was 450 s 3 for the best technology. AB-8 to determine the total flavonoids wax flower adsorption resin. The optimum static adsorption conditions were as follows: temperature 30 ℃, adsorption time 3 h; optimum dynamic desorption conditions were: flow rate 2.6 ml / min, pH value of the sample solution 4.3, 30 ml 70% ethanol. Compared with the solvent extraction method, the extraction time of total flavonoids from Radix Plum was shortened from 20 h to 450 s by microwave method, the extraction rate increased from 79.86% to 92.23%, and the separation effect of total flavonoids from Radix Plum Bl. low. [Conclusion] The microwave and macroporous resin can be widely used in the industrialization of extraction of total flavonoids from