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目的研究一氧化氮（ＮＯ）在血吸虫直肠肉芽肿发病机制中的作用。方法采用腹部敷贴法建立血吸虫病兔模型，ＳＰ免疫组化法分析结构型和诱导型一氧化氮合成酶（ｃＮＯＳ和ｉＮＯＳ）在正常兔和血吸虫病兔直肠壁的表达与分布。结果正常兔直肠壁ｃＮＯＳ和ｉＮＯＳ呈阴性反应，血吸虫病兔直肠壁为强阳性，ｃＮＯＳ主要分布在粘膜毛细血管和粘膜下血管壁；ｉＮＯＳ不仅见于上述血管壁，而且见于虫卵、虫卵肉芽肿周围、纤维结缔组织中、炎性细胞之间。结论ＮＯ在血吸虫直肠肉芽肿发病机制中具有重要作用
Objective To study the role of nitric oxide (NO) in the pathogenesis of rectal granuloma in schistosomiasis. Methods Rabbit models of schistosomiasis were established by abdominal plaster. The expression and distribution of structural and inducible nitric oxide synthase (cNOS and iNOS) in rectal wall of normal rabbits and schistosomiasis rabbits were analyzed by SP immunohistochemistry. Results Normal rabbit cNOS and iNOS negative reactivity, schistosomiasis rabbit rectum wall is strongly positive, cNOS mainly in the mucosal capillaries and submucosal blood vessel wall; iNOS not only seen in the above vascular wall, but also found in eggs, eggs, granulomas Around, fibrous connective tissue, between inflammatory cells. Conclusion NO plays an important role in the pathogenesis of rectal granuloma in schistosomiasis