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目的:探讨自体移植脾组织能否降低 OPSI 的发病率。方法:对再生脾本身进行研究,32只 SD 雌性幼龄大鼠分为两组,对照组16只,自体脾组织移植组16只。术后22周检测脾细胞对羊红细胞(SRBC)的抗体反应及对有丝分裂原增殖反应,并用 SDS-聚丙烯酰胺凝胶电泳分析其蛋白组成。结果显示:移植组脾细胞对 SRBC 抗体生成无明显改变(P<0.01),T 细胞对刀豆素 A增殖反应明显减弱,而 B 细胞对脂多糖反应正常。说明移植睥组织经再生后,B 细胞数量和功能正常,而 T 细胞功能发生了改变。蛋白电泳显示自体移植脾细胞与正常脾细胞相比,能产生更明显的19000带,而16000、29000带缺失。结论:再生脾与正常脾组织在分子水平和功能上存在差异,脾切除后仅行自体脾组织移植还不能达到预防 OPSI 的目的。
Objective: To investigate whether autologous transplantation of spleen tissue can reduce the incidence of OPSI. Methods: The spleen itself was studied. Thirty-two female SD rats were divided into two groups: control group (16) and autologous spleen group (16). At 22 weeks after operation, the splenocytes response to SRBC and the proliferative response to mitogen were detected. The protein composition of spleen cells was analyzed by SDS-polyacrylamide gel electrophoresis. The results showed that there was no significant change (P <0.01) in the generation of SRBC antibody in the spleen cells in the transplantation group, the proliferative response of the T cells to the concanavalin A was obviously weakened, and the B cells reacted normally to the lipopolysaccharide. Explanations of transplanted 睥 tissue after regeneration, the number and function of normal B cells, and T cell function has changed. Protein electrophoresis showed that compared with normal splenocytes, autologous transplantation spleen cells produced more obvious 19000 bands, while 16000 and 29000 bands were deleted. CONCLUSION: There are differences in molecular level and function between regenerative spleen and normal spleen tissue. After splenectomy, autologous spleen transplantation alone can not achieve the purpose of preventing OPSI.