论文部分内容阅读
目的 :探讨小胶质细胞在慢性脑灌注不足脑损害中的作用。方法 :70只老龄大鼠持久性双侧颈总动脉结扎 ( 2VO) ,其中12只接受环孢霉素A(CsA)胃灌治疗。免疫组化法单抗OX18、OX6标记小胶质细胞。实验研究为持久性 2VO 1～ 4月。结果 :持久性 2VO 1月皮层、海马和白质处均有明显的小胶质细胞活动。 1～ 4月 ,皮层和白质处小胶质细胞的活动增多 ,而海马处小胶质细胞的活动则减少 ,同时脑损害加重。CsA治疗后小胶质细胞的活动明显减少 ,脑损害明显减轻。结论 :小胶质细胞的活动在大鼠慢性脑灌注不足脑损害 ,特别是大脑白质损害中起重要作用 ,CsA可抑制大鼠慢性脑灌注不足脑内小胶质细胞的活动 ,从而减轻了脑损害
Objective: To investigate the role of microglia in brain damage caused by chronic cerebral hypoperfusion. Methods: Totally 70 aged rats were subjected to permanent bilateral common carotid artery ligation (2VO). Twelve of them received cyclosporine cyclosporine A (CsA) gastric perfusion. Immunohistochemistry monoclonal antibody OX18, OX6 labeled microglia. Experimental study for the persistence of 2VO 1 ~ April. Results: The persistent 2VO in January cortex, hippocampus and white matter were obvious microglial activity. From January to April, the activity of microglia in the cortex and white matter increased, while the activity of microglia in the hippocampus decreased while the brain damage increased. The activity of microglial cells after CsA treatment was significantly reduced, and the brain damage was significantly reduced. CONCLUSION: The microglial activity plays an important role in the brain damage of chronic cerebral hypoperfusion in rats, especially in the white matter damage of the brain. CsA can inhibit the activity of microglia in the brain of chronic cerebral hypoperfusion in rats, damage