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目的:研究莪芪抗瘤方对二乙基亚硝胺(DEN)诱导肝癌模型鼠肿瘤微环境的调控机制。方法:建立DEN诱导大鼠肝癌模型,造模4周后,70只大鼠随机分成7组,空白组,模型组,顺铂治疗组,单纯祛邪中药组,莪芪抗瘤方组随机分为高、中、低剂量组。每组10只。空白组正常喂养,模型组每日12.5 mg/kg体重维持DEN摄入量喂养,莪芪抗瘤方中剂量组、单纯祛邪中药组、顺铂治疗组,分别以8 mL/kg体重剂量灌药;莪芪抗瘤方高、低剂量组分别以12 mL/kg体重、4 mL/kg体重剂量灌药。16周实验结束,测定大鼠血清白细胞介素-1β(IL-1β)、白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)的表达。结果:莪芪抗瘤方组IL-1β与单纯祛邪中药组、空白组比较,差异有统计学意义(P<0.05),与顺铂治疗组比较,差异无统计学意义(P>0.05);莪芪抗瘤方组IL-2与单纯祛邪中药组比较,差异有统计学意义(P<0.05);莪芪抗瘤方组IL-2、TNF-α与空白组、顺铂治疗组比较,差异无统计学意义(P>0.05)。结论:莪芪抗瘤方能抑制肝癌大鼠模型血清中IL-1β表达,抑制肿瘤组织血管的形成和浸润,促进IL-2的分泌,增强免疫力,抑制肿瘤的形成,在肝癌形成过程中具有一定的延缓和保护作用
Objective: To investigate the regulatory mechanism of Curcuma Anticancer Decoction on the tumor microenvironment of hepatocellular carcinoma model induced by diethylnitrosamine (DEN). Methods: DEN-induced rat model of hepatocellular carcinoma was established. After 4 weeks of modeling, 70 rats were randomly divided into 7 groups. The rats in the blank group, model group, cisplatin-treated group, For high, medium and low dose group. Each group of 10. The rats in the blank group were fed with normal diet. The rats in the model group were given 12.5 mg / kg body weight daily to maintain the intake of DEN. The middle dose of Curcuma Anticancer prescription, Quxu Chinese medicine group and cisplatin treatment group were fed with 8 mL / Medicine; Curcuma anti-tumor side high and low dose groups were 12 mL / kg body weight, 4 mL / kg body weight dose of drug. At the end of 16 weeks, the levels of serum IL-1β, IL-2 and TNF-α were determined. Results: Compared with the traditional Chinese medicine group and the blank group, the difference was statistically significant (P <0.05), but there was no significant difference between the two groups (P> 0.05) (P <0.05). IL-2 and TNF-α in Curcuma Anticancer group was significantly higher than that in blank group and Cisplatin-treated group The difference was not statistically significant (P> 0.05). Conclusion: Curcuma anticoagulant can inhibit the expression of IL-1β in sera of rat model of hepatocellular carcinoma, inhibit the formation and infiltration of tumor blood vessels, promote the secretion of IL-2, enhance the immunity and inhibit the formation of tumor. Has a certain delay and protection