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目的观察2型糖尿病大鼠心肌凋亡相关蛋白Bax、Bcl-2和Caspase-3的表达变化及叶酸、维生素B12的干预作用。方法 6周龄SD大鼠60只,糖尿病造模成功后,随机分为对照组(A组)、模型组(B组)、叶酸和维生素B12干预组(C组)。12周后取血检测各组同型半胱氨酸(Hcy)、叶酸、维生素B12的水平;取大鼠心肌组织,免疫组化法检测Bax,Bcl-2,Caspase-3的表达,并用计算机图像分析系统测平均灰密度值。结果 12周后B组Hcy明显增高,与A组和C组比较,差异均有统计学意义(P<0.01),C组叶酸、维生素B12与A组和B组比较,差异均有统计学意义(P<0.01);心肌组织中,B组Bax、Caspase-3的表达明显高于A组(P<0.01),Bcl-2的表达明显低于A组(P<0.01);C组Bax、Caspase-3的表达较B组明显下降(P<0.01),Bcl-2的表达明显高于B组(P<0.01);A组与C组Bax、Caspase-3、Bcl-2的表达差异无统计学意义(P>0.05)。结论 2型糖尿病大鼠血液中Hcy的升高,可能在促进心肌细胞凋亡过程中发挥重要作用,而叶酸、维生素B12的干预可以有效缓解病变进程。
Objective To investigate the changes of myocardial apoptosis-related proteins Bax, Bcl-2 and Caspase-3 in type 2 diabetic rats and the intervention of folic acid and vitamin B12. Methods Sixty SD rats aged 6 weeks were randomly divided into control group (group A), model group (group B), folic acid and vitamin B12 intervention group (group C) after successful diabetes mellitus. The levels of homocysteine (Hcy), folic acid and vitamin B12 in each group were measured after 12 weeks. The expression of Bax, Bcl-2 and Caspase-3 in myocardium of rats was detected by immunohistochemistry. Analyze the system to measure the average ash density value. Results After 12 weeks, the Hcy in group B was significantly higher than that in group A and group C (P <0.01). The differences of folic acid and vitamin B12 in group C between group A and group B were statistically significant (P <0.01). The expression of Bax and Caspase-3 in group B was significantly higher than that in group A (P <0.01), while the expression of Bcl-2 was significantly lower in group B than that in group A The expression of Caspase-3 in B group was significantly lower than that in B group (P <0.01), and the expression of Bcl-2 was significantly higher than B group (P <0.01). There was no significant difference in Bax, Caspase-3 and Bcl- Statistical significance (P> 0.05). Conclusion The increase of Hcy in the blood of type 2 diabetic rats may play an important role in the promotion of cardiomyocyte apoptosis. However, the intervention of folic acid and vitamin B12 can effectively alleviate the pathological process.