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1975年以来,我们用红色和蓝色有机染料分别对有生活力的种子和已死亡的种子,进行浸泡染色处理,发现某些有机染料,不能渗透到有生命的胚细胞里,而能够顺利地渗透到已死亡了的细胞里,并使细胞质膜、细胞质和细胞核染上颜色。这是因为生活着的细胞,其细胞质膜具有选择透性,完全能够阻碍有机染料的渗入,而死亡了的细胞则失去了这种选择吸收能力,经浸泡染色处理后就显现出一定的颜色。根据这个道理,用0.1%的酸性复红或靛蓝试液进行,
Since 1975, we dyed and treated viable seeds and dead seeds with red and blue organic dyes, respectively, and found that certain organic dyes did not penetrate into living germ cells and smoothly Penetrates into dead cells and colors the plasma membrane, cytoplasm and nucleus. This is because living cells, the plasma membrane of the cell with a selective permeability, can hinder the infiltration of organic dyes, while the dead cells have lost this selective absorption capacity, after immersion dyeing process showed a certain color. According to this principle, with 0.1% acid complex red or indigo test solution,