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本研究克隆了白桦(Betula platyphylla)UVR8基因,命名为BpUVR8(Gen Bank:AHY02156)。生物信息学分析表明该基因全长1 326 bp,编码441个氨基酸,为稳定性蛋白,不存在信号肽,具有2个跨膜区。二级结构分析表明该蛋白属于混合型。三级结构预测结果显示,BpUVR8与AtUVR8蛋白结构具有较高相似性,均由7个β螺旋组成片层结构。BpUVR8表达模式分析表明,其主要在白桦叶片中表达,且8月份表达量最高;紫外诱导6 h,其转录表达水平上调约2倍;同时,非生物胁迫以及信号诱导在一定时间内也能影响该基因的转录表达。本文为研究白桦对UV-B信号的响应以及对UVR8功能的深入研究提供参考。
In this study, the UVR8 gene of Betula platyphylla was cloned and named BpUVR8 (Gen Bank: AHY02156). Bioinformatics analysis showed that the gene was 1 326 bp in length, encoding 441 amino acids. It was a stable protein with no signal peptide and two transmembrane regions. Secondary structure analysis shows that the protein is mixed. The results of the tertiary structure prediction showed that the structure of BpUVR8 and AtUVR8 protein were highly similar and consisted of seven β-helices. The analysis of BpUVR8 expression pattern showed that it was mainly expressed in the leaves of Betula platyphylla, and the expression level was the highest in August. The expression level of BpUVR8 was up-regulated by about 2-fold at 6 h after UV induction. At the same time, abiotic stress and signal induction also affected in certain time Transcriptional expression of this gene. In this paper, we will provide a reference for studying the response of Betula platyphylla to UV-B signal and its further study on the function of UVR8.