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目的探讨瘦素基因型与哈萨克族肥胖儿童的关系。方法按照肥胖儿童诊断标准,选择乌鲁木齐周边地区96例哈萨克族肥胖患儿(肥胖组)和81例健康对照儿童(非肥胖组),空腹12 h后抽取2组儿童外周静脉血5 mL,常规提取基因组DNA,应用限制性片段长度多态性方法检测其C2549A瘦素基因型,同时检测各组空腹血脂水平,采用放射免疫法测定其空腹血浆瘦素和胰岛素水平。并测量各组儿童身高、体质量。结果 177例受试对象中,AA、AC、CC基因型频率分别为6.8%、52.5%、40.7%。A等位基因频率为33.0%,C等位基因频率为67.0%;瘦素、胰岛素等各项指标在AA+AC组与CC组间及不同性别间比较差异无统计学意义;肥胖组患儿与非肥胖组儿童比较,除高密度脂蛋白、载脂蛋白A、载脂蛋白B外,其血清三酰甘油、总胆固醇、低密度脂蛋白、瘦素、胰岛素水平肥胖患儿均明显高于非肥胖儿童。结论在哈萨克族儿童中存在瘦素基因A2549C多态性;瘦素基因多态性与哈萨克族儿童肥胖无关。
Objective To investigate the relationship between leptin genotypes and Kazakh obese children. Methods According to the diagnostic criteria of obese children, 96 Kazak obese children (obese group) and 81 healthy control children (non-obese group) were selected in the peripheral area of Urumqi. After 12-hour fasting, 5 mL of peripheral venous blood was extracted from 2 children and routinely extracted Genomic DNA was detected by restriction fragment length polymorphism (RFLP). The C2549A leptin genotypes were detected. Fasting plasma lipids were measured at the same time. The levels of fasting plasma leptin and insulin were determined by radioimmunoassay. The height and body mass of children in each group were measured. Results Among 177 subjects, the frequencies of AA, AC and CC genotypes were 6.8%, 52.5% and 40.7%, respectively. A allele frequency was 33.0%, C allele frequency was 67.0%; leptin, insulin and other indicators in the AA + AC group and CC group and gender differences between the groups was not statistically significant; obese children Compared with non-obese children, except high-density lipoprotein, apolipoprotein A and apolipoprotein B, serum triglyceride, total cholesterol, low density lipoprotein, leptin and insulin in obese children were significantly higher than those in obese Non-obese children. Conclusion Leptin gene A2549C polymorphism exists in Kazakh children; leptin gene polymorphism has nothing to do with Kazakh children obesity.