目的了解不同稀释液制备的试验菌菌悬液的存活时限,为微生物实验室的菌悬液制备及使用提供参考。方法参照2010年版《中国药典》微生物限度检查的菌悬液制备,用0.9%氯化钠溶液、0.1%蛋白胨水溶液、p H值为7的氯化钠-蛋白胨缓冲液作为稀释液,分别对6种试验菌制备成含菌数约100 cfu/ml的菌悬液,于4℃保存,并进行活菌计数。结果黑曲霉菌悬液和枯草芽孢杆菌的芽孢菌悬液在3种稀释液中3个月的活菌数相对稳定;白色念珠菌在p H值为7的氯化钠-蛋白胨缓冲液中1个月的活菌数相对稳定;大肠埃希菌、枯草芽孢杆菌、铜绿假单胞菌、白色念珠菌等在p H值为7的氯化钠-蛋白胨缓冲液中的稳定性优于另外2种稀释液。结论采用适宜的稀释液制备试验菌的菌悬液,可提高其稳定性,延长存活时限,从而提高工作效率,节约实验成本,更适应微生物实验室的工作需要。 Objective To understand the survival time of test bacterial suspension prepared by different dilutions and provide reference for the preparation and use of bacterial suspension in microbial laboratory. Methods According to the microbial suspension of Chinese Pharmacopoeia (2010 edition), the bacterial suspensions were prepared with 0.9% sodium chloride solution, 0.1% peptone solution and sodium chloride-peptone buffer with pH 7 as diluent. The test bacteria were prepared into bacterial suspensions with a bacterial count of about 100 cfu / ml, stored at 4 ° C, and viable count was performed. Results The number of viable cells in the three dilutions of Aspergillus niger and Bacillus subtilis was relatively stable for 3 months; Candida albicans was stable in sodium chloride-peptone buffer with pH 7 The number of viable cells in a month was relatively stable; the stability of Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans, etc. in sodium chloride-peptone buffer with pH 7 was better than that of the other 2 Dilutions. Conclusion The preparation of bacterial suspension of test bacteria with appropriate dilution can improve the stability and extend the survival time, so as to improve the working efficiency, save the experimental cost and adapt to the work needs of the microbiological laboratory.