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目的:通过建立兔股骨缺损的动物实验模型,对采用等温化学气相沉积法和等离子喷涂技术所制备的石墨化炭/炭复合材料+羟基磷灰石涂层(C/C+HA)复合骨植入材料进行骨植入实验的的生物相容性进行评价,探索该复合材料作为植入机体骨组织的可行性依据。方法:采用骨科钻在实验动物股骨髁上钻孔的方法建立骨缺损的动物实验模型,将待研究比较的实验材料分别植入实验动物的股骨髁内,持续观察8周,在术后第2、4、8周时应用X线照片、组织学染色和扫描电镜技术,分别观察所研究材料在机体内对骨缺损愈合及其对机体的影响,进行组间比较和相关性分析。结果:石墨化炭/炭复合材料+羟基磷灰石涂层(C/C+HA)复合骨植入材料的骨植入实验生物相容性良好,材料与骨组织结合牢固,界面中成骨细胞生长明显,且炭颗粒脱落现象少,未见炎症细胞浸润。植入动物体内的材料在植入期未引起机体局部的炎症浸润反应且表面脱落的碳颗粒在机体组织中也未引起局部严重的炎症反应。在实验动物植入材料后的连续8周观察期中,组织学观察显示:表面涂有HA的炭/炭复合材料对骨组织形态改建上表现良好,其与骨组织接界处所形成的纤维结缔组织膜层厚度明显比未涂HA的材料要小,与骨组织结合更为紧密和牢固;碳颗粒出现脱落游离的现象明显减少。结论:在炭/炭复合材料表面涂以HA生物涂层对骨的形态改建和促进骨小梁生长等方面具有良好的作用,是一种具有发展潜力的骨修复材料。
OBJECTIVE: To establish an animal experimental model of rabbit femoral defect by means of isothermal chemical vapor deposition and plasma spray coating technology. The graphitized carbon / carbon composite + hydroxyapatite coating (C / C + HA) The biocompatibility of the materials into the bone implantation experiment was evaluated to explore the feasibility of the composite as a bone implantable body. Methods: The experimental model of bone defect was established by drilling the femoral condyle of the experimental animal with an orthopedic drill. The experimental materials to be compared were implanted into the femoral condyle of the experimental animals for 8 weeks. After the second At the 4th and 8th week, X-ray pictures, histological staining and scanning electron microscopy were used to observe the effect of the materials on the healing of bone defects and their effects on the body. The comparison and correlation analysis between the groups were made. Results: The experimental results showed that the biocompatibility of C / C + HA composite bone implants was good, Significant cell growth, and charcoal particles shedding less, no inflammatory cell infiltration. Materials implanted in animals did not cause local inflammatory infiltration during implantation and surface detachment of carbon particles did not cause local severe inflammation in body tissues. Histological observation showed that HA-coated carbon / carbon composites performed well on morphological remodeling of the bone tissue at the 8-week observation period after implantation of the experimental animals. The fibrous connective tissue formed at the interface with the bone tissue The thickness of the film is obviously smaller than that of the non-HA coated material, and it is more tightly and firmly bonded with the bone tissue. The phenomenon of detachment of carbon particles is obviously reduced. CONCLUSION: HA coated on the surface of carbon / carbon composite has a good effect on the morphological modification of bone and the growth of trabecular bone. It is a potential bone repair material.