[Objective] To determine the optimal extraction technology of total alkaloids from RADIX ACONITI LATERALIS PRAEPARATA.[Method] With dry extracts rate,and the purity and extraction rate of total alkaloids as the indices,effects of extraction solvent,extraction method,extraction temperature,extraction time,grind degree,solid-liquid ratio and extraction times on the extraction rate of total alkaloids were studied.And the optimal extraction technology was selected.[Conclusion] The optimal extraction technology was as follows:RADIX ACONITI LATERALIS PRAEPARATA crude powder was added into anhydrous ethanol with the ratio of 1:10(g/ml)under 15 ℃.After cold leaching for 3 times with each time for 1 d,the dry extracts rate,extraction rate of total alkaloids,and purity of total alkaloids were 3.15%,0.64% and 20.32%,respectively.[Conclusion] This method was efficient,low toxic,economical,convenient and can be used for industrial production. [Objective] To determine the optimal extraction technology of total alkaloids from RADIX ACONITI LATERALIS PRAEPARATA. [Method] With dry extracts rate, and the purity and extraction rate of total alkaloids as the indices, effects of extraction solvent, extraction method, extraction temperature, extraction time, grind degree, solid-liquid ratio and extraction times on the extraction rate of total alkaloids were studied. And the optimal extraction technology was selected. [Conclusion] The optimal extraction technology was as follows: RADIX ACONITI LATERALIS PRAEPARATA crude powder was added into absolute ethanol with the ratio of 1:10 (g / ml) under 15 ° C. After cold leaching for 3 times with each time for 1 d, the dry extract rate, extraction rate of total alkaloids, and purity of total alkaloids were 3.15 %, 0.64% and 20.32%, respectively. [Conclusion] This method was efficient, low toxic, economical, convenient and can be used for industrial production.