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用~(32)P后标记法,HPLC及PEI·TLC同谱层析等方法对DNA-苯醌(BQ)加合物进行了研究,并对其中一个主要加合物进行了碱基定位,确证该斑点系BQ与分子DNA上的2′-脱氧-3′-磷酸胞嘧啶作用形成的加合物(dC-BQ加合物)。
The DNA-benzoquinone (BQ) adduct was studied by ~ (32) P post-labeling method, HPLC and PEI · TLC, and the base adduct of one of the major adducts was located. This dot was confirmed to form an adduct (dC-BQ adduct) of BQ with 2’-deoxy-3’-phosphocytosine on molecular DNA.