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目的探讨胰岛素受体底物-2的Gly1057Asp基因多态性与颈动脉粥样硬化性狭窄的相关性。方法选择96例行颈部血管彩超示颈动脉狭窄≥50%的北方汉族人为实验组以及同期79例体检健康者作为对照组,通过聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术检测所有入选患者的Gly1057Asp基因多态性,比较2组的等位基因频率、基因型频率以及Gly1057Asp基因多态性对颈动脉狭窄的影响。结果实验组中G等位基因频率(78.6%)明显高于对照组(68.4%)(P<0.05);实验组中G/A+A/A基因型频率(36.5%)明显低于对照组(51.9%)(P<0.05)。携带等位基因G的个体患有颈动脉粥样硬化性狭窄的风险是非等位基因G携带者的1.99倍(OR=1.994,95%CI=1.068~3.761)。结论胰岛素受体底物-2的Gly1057Asp基因多态性可能与颈动脉粥样硬化性狭窄有关,等位基因G可能是颈动脉粥样硬化性狭窄的独立危险因素。
Objective To investigate the relationship between Gly1057Asp gene polymorphism of insulin receptor substrate-2 and carotid atherosclerosis. Methods Ninety-six Northern Han Chinese subjects with neck stenosis≥50% were selected as the experimental group and 79 healthy subjects as control group by cervical vascular color Doppler ultrasound examination. PCR-restriction fragment length polymorphism (PCR- RFLP) technique was used to detect Gly1057Asp polymorphism in all patients. The allele frequency, genotype frequency and Gly1057Asp polymorphism in the two groups were compared. Results The frequency of G allele (78.6%) in the experimental group was significantly higher than that in the control group (68.4%) (P <0.05). The frequency of G / A + A / A genotype in the experimental group was significantly lower than that in the control group (51.9%) (P <0.05). Individuals carrying allele G were 1.99 times more likely to have carotid atherosclerotic stenosis than non-allele G carriers (OR = 1.994, 95% CI = 1.068-3.761). Conclusion Gly1057Asp gene polymorphism of insulin receptor substrate-2 may be related to carotid atherosclerosis. Allele G may be an independent risk factor for carotid atherosclerosis.