论文部分内容阅读
本文作者采用聚合酶链式反应（ＰＣＲ），从ＨＩＶ－１ｃＤＮＡ克隆ＢＨ１０中扩增出Ｐｒ４２ｇａｇ的基因片段，经适当的限制性内切酶消化后插入到杆状病毒转移载体ｐＢａｃＰＡＫ９中，使其处于多角蛋白启动子的控制之下，构成重组转移载体ｐＢａｃＧＡＧ４２。酶切鉴定结果与预期的一致。再用载体上多克隆位点两翼的引物对连接处进行序列分析，结果表明外源基因处于多角蛋白启动子的控制下，且成功地引入了起始码和终止码。
In this study, polymerase chain reaction (PCR) was used to amplify the gene fragment of Pr42gag from HIV-1 cDNA clone BH10 and inserted into the baculovirus transfer vector pBacPAK9 after appropriate restriction endonuclease digestion, Under the control of the polyhedrin promoter, a recombinant transfer vector pBacGAG42 was constructed. Restriction enzyme digestion results and expectations. Sequence analysis showed that the exogenous gene was under the control of polyprotein promoter, and the start codon and stop codon were successfully introduced.