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为了研究烟草根际土壤细菌多样性,了解其细菌群落的组成和结构,从而为健康植烟土壤生态系统的构建和保持提供理论依据和指导。以2014年四川凉山州健康烟株现蕾期根际土壤为研究样本,利用Illumina平台Miseq高通量测序技术对烟草根际土壤细菌16S rDNA进行高通量测序分析。结果表明:烟草根际土壤微生物多样性水平较高,不同样品间主要菌落分布结构相对稳定。在门分类水平上,共检测分类25个菌门,其中变形菌门占有明显优势(39.04%~53.71%),其次为拟杆菌门、酸杆菌门、疣微菌门;在属分类水平上,共检测分类576类菌属,鞘脂单胞菌属、Flavisolibacter、Ohtaekwangia为烟草根际土壤细菌明显优势菌属。大部分主要菌群之间存在明显正相关关系,对烟草根际土壤细菌影响较大的环境因子为土壤速效氮、含水量和pH。运用宏基因组学16S rDNA测序技术获取的烟草根际土壤细菌信息较为丰富,为健康植烟土壤机制研究提供新的理论参考。
In order to study the bacterial diversity in rhizosphere soils and to understand the composition and structure of bacterial communities, this study will provide a theoretical basis and guidance for the construction and maintenance of healthy tobacco-planting soil ecosystems. The rhizosphere soils of healthy tobacco plants in Liangshan Prefecture of Sichuan Province in 2014 were taken as samples to analyze the 16S rDNA of rhizobacterial soil rhizobacteria using Illumina platform Miseq high-throughput sequencing. The results showed that the microbial diversity in tobacco rhizosphere soil was high, and the distribution of the main colonies in different samples was relatively stable. On the door classification level, were classifier 25 phylum, wherein Proteobacteria clear advantage (39.04% - 53.71%), followed by Bacteroides door, acidobacteria, Verrucomicrobia door; on Taxonomy level, A total of 576 strains of bacteria, Sphingomonas, Flavisolibacter and Ohtaekwangia were identified as dominant bacterial species in tobacco rhizosphere soil. There was a significant positive correlation between most of the major microflora and the environmental factors affecting soil rhizosphere bacteria were soil available nitrogen, water content and pH. The rhizosphere soil bacteria obtained by metagenomic 16S rDNA sequencing technology is rich in information, which provides a new theoretical reference for the research on the mechanism of tobacco planting soil health.