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目的探讨尼如哈优化剂对溃疡性结肠炎相关癌的防治作用。方法 98只Balb/c小鼠随机分空白组、模型组、优化剂高、中、低剂量治疗组,传统剂治疗组,柳氮磺吡啶治疗组7组,每组10只;正常组除外,各组以AOM/DSS复合法复制模型,从第9周开始模型组予等剂量生理盐水灌肠,治疗各组分别予相应的药液进行灌肠治疗,共11周。实验第20周末将全部小鼠处死取全结肠,肉眼及光镜下观察其结肠组织形态学改变及异常隐窝的变化。期间每周一次观察小鼠一般情况。结果模型组小鼠结肠肉眼改变以散在、多发性息肉状隆起性病变为主。病理改变以低级别上皮内瘤变和/或高级别上皮内瘤变为主;低、中剂量组肉眼改变均以单发或少数息肉状隆起性病变为主。镜下以增生性息肉改变为主;高剂量组、柳氮磺吡啶组肉眼改变均以结肠黏膜充血、水肿为主。镜下改变均以腺体增生为主,未见溃疡及明显的异常隐窝及上皮内瘤变。与模型组比较有极显著差异(P<0.01)。空白组小鼠情况良好,摄食量、大便正常;模型组小鼠体质量逐渐减轻,倦怠拱背、有黏液血便,并出现脱肛;尼如哈治疗各组小鼠体质量和食量不同程度增加、血便次数明显减少。结论蒙医尼如哈优化剂可用于防治溃疡性结肠炎相关癌。
Objective To investigate the preventive and therapeutic effects of Nicorandil on the cancer related to ulcerative colitis. Methods 98 Balb / c mice were randomly divided into blank group, model group, high, medium and low dose treatment groups, traditional medicine treatment group and sulfasalazine treatment group (n = 10) Each group was replicated by AOM / DSS method. From the 9th week, the model group was given the same dose of saline enema. The treatment groups were given the corresponding liquid for enema treatment for 11 weeks. At the end of the experiment, all the mice were sacrificed at the end of the experiment to take the whole colon. The morphological changes of colonic tissue and abnormal crypts were observed under naked eye and light microscope. During the week to observe the general situation of mice. Results The macroscopic changes of the colon in the model group were dominated by scattered and multiple polypoid lesions. Pathological changes with low-grade intraepithelial neoplasia and / or high-grade intraepithelial neoplasia mainly; low and middle dose group of macroscopic changes were single or few polypoid lump lesions. Microscopic changes mainly to proliferative polyps; high-dose group, sulfasalazine group were macroscopic colonic mucosal hyperemia, edema-based. Microscopic changes were mainly glandular hyperplasia, no ulcers and obvious abnormal crypts and intraepithelial neoplasia. Compared with model group, there was significant difference (P <0.01). The mice in the blank group were in good condition, the food intake and the stool were normal. The body weight of the mice in the model group gradually decreased, the backache of the model group had mucous bloody stools and the rectal prolapse appeared. The body weight and appetite of the mice in each group were increased to some extent, Bloody stools decreased significantly. Conclusion Mengni Neruha can be used to prevent and treat ulcerative colitis-related cancers.