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从人肝癌细胞(BEL-7402)高度免疫过的绵羊的淋巴器官中抽提的肝癌“免疫”RNA,和正常人外周血淋巴细胞温育后,在体外能介导肝癌细胞的免疫细胞溶解。用5-~(125)碘-2′-脱氧尿嘧啶核苷标记人肝癌靶细胞的微量细胞毒性试验进行测定时观察到,正常人淋巴细胞经肝癌“免疫”RNA处理后,其细胞毒性指数在16次实验中有10次有显著增高,但是,在与对照RNA温育后其细胞毒性指数(除一次实验外)均未出现有显著意义的变化。肝癌“免疫”RNA在用RNA酶降解后,传递细胞毒性免疫反应的活性即消失,而用DNA酶或灰链霉菌蛋白酶处理,却仍保持这种传递能力。由此证明,异种“免疫”RNA能够把针对人肝癌细胞表面抗原的细胞免疫性传递给正常人淋巴细胞。
Liver cancer “immune” RNA extracted from lymphoid organs of sheep highly immunized with human hepatoma cells (BEL-7402), after incubation with normal human peripheral blood lymphocytes, mediates immune cell lysis of hepatoma cells in vitro. The cytotoxicity of normal human lymphocytes treated with “immune” RNA of liver cancer was observed in the cytotoxicity assay using 5 ~ (125) iodo-2’-deoxyuridine labeled human hepatoma target cells. The cytotoxicity index There were 10 significant increases in 16 experiments, but no significant change in cytotoxicity index (except one experiment) occurred after incubation with control RNA. Liver cancer “immune” RNA is degraded by RNase, the activity of delivering a cytotoxic immune response disappears, whereas it is maintained by DNase or Streptomyces protease. This demonstrates that heterologous “immune” RNA can deliver cellular immunity against human hepatoma cell surface antigens to normal human lymphocytes.