一.导言 1975年Kohlor和Milstein在自然杂志发表题为“分泌预定特异性抗体的融合细胞持续培养”一文,指出在体外可将这些细胞大规模培养以提供特异性抗体,对医学和工业可能有应用价值。在单克隆抗体被应用的12年中,它们对医学基础研究已经产生了巨大的影响,现正进入临床领域,将给临床上疾病的诊断、治疗、预后和研究发病机理带来深远的影响。所谓单克隆抗体(Monoclonal Antibody缩写:MAb)是指由一种杂交瘤细胞产生并 I. Introduction Kohlor and Milstein published a paper in Nature in 1975 titled “Sustained Culturing of Conjugated Cells Secreting Specific Antibodies” that states that these cells can be cultured on a large scale in vitro to provide specific antibodies that may be of interest to both medicine and industry Value. Monoclonal antibodies have been applied for 12 years, they have a fundamental medical research has had a huge impact, is entering the clinical field, clinical diagnosis and treatment of diseases, prognosis and study the pathogenesis of far-reaching impact. The so-called monoclonal antibody (Monoclonal Antibody abbreviation: MAb) refers to a hybridoma cells and