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对弯曲乳杆菌Lactobacillus crispatus T79-3和T90-1、詹氏乳杆菌Lactobacillus jensenii T118-3和T231-1四株乳杆菌对金黄色葡萄球菌生长的抑制效果以及抑菌成分进行了分析,比较乳杆菌排除、竞争、置换3种不同作用方式对金黄色葡萄球菌粘附HeLa细胞的抑制作用。结果表明4株乳杆菌皆能抑制金黄色葡萄球菌的生长及其粘附HeLa细胞的能力,分析发现4株乳杆菌发挥抑制作用的主要成分是有机酸,同时比较分析乳杆菌3种不同作用方式发现它们对金黄色葡萄球菌粘附HeLa细胞的抑制效果不同,其中,排除作用方式效果最好。另外,乳杆菌对金黄色葡萄球菌粘附HeLa细胞的抑制作用具有浓度依赖性,随着乳杆菌浓度增大,抑制作用增强并逐渐达到饱和。4株乳杆菌中,T79-3粘附能力最强,对金黄色葡萄球菌的抑制作用最强,排除作用方式抑制金黄色葡萄球菌粘附HeLa细胞作用效果较好,提示乳杆菌T79-3有可能作为益生菌防治妇女泌尿生殖道感染。
The inhibitory effects of Lactobacillus crispatus T79-3 and T90-1, Lactobacillus jensenii T118-3 and T231-1 four strains of Lactobacillus on the growth of Staphylococcus aureus and antibacterial components were analyzed, Bacteriostatic elimination, competition, replacement of three different modes of action of Staphylococcus aureus adhesion HeLa cells. The results showed that all four strains of Lactobacillus could inhibit the growth of Staphylococcus aureus and its ability to adhere to HeLa cells. The main component of the four strains of Lactobacillus was the organic acids. Three different modes of action of Lactobacillus were also analyzed Found that they inhibit the adhesion of Staphylococcus aureus HeLa cells, of which the best way to exclude. In addition, Lactobacillus on Staphylococcus aureus adhesion to HeLa cells in a concentration-dependent manner, with the concentration of Lactobacillus increased inhibition and gradually reached saturation. Among the four strains of Lactobacillus, T79-3 had the strongest adhesion ability, the strongest inhibitory effect on Staphylococcus aureus, and the elimination mode of action inhibited the adhesion of Staphylococcus aureus to HeLa cells, suggesting that Lactobacillus T79-3 had Probiotics may prevent and treat genitourinary infections in women.