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以百日草为试验材料,利用同源基因克隆法克隆到百日草几丁质酶基因片段和actin基因片段,分别用链霉菌769发酵液和百日草黑斑病病菌悬浮液处理百日草植株,通过半定量RT-PCR检测了不同处理的几丁质酶基因表达量的差异。结果表明,链霉菌769发酵液和百日草黑斑病病菌悬浮液均能诱导百日草几丁质酶基因的表达,其最大表达量分别在接种后24 h和6 h。百日草黑斑病病菌优先诱导植株体内几丁质酶的表达,而链霉菌769的诱导滞后。当用病原菌处理36 h后喷施链霉菌769发酵液,链霉菌769对几丁质酶基因的诱导表达高峰出现在喷施后的36 h,比单独喷施的诱导表达推迟了12 h,暗示了生防菌链霉菌769可以诱导植物的系统诱导抗性,且和百日草黑斑病病菌引发了不同的信号转导通路,以减轻由病原菌侵染引发的植物病害。
Taking Zinnia elegans as the experimental material, cloned the zinnia chitinase gene fragment and the actin gene fragment by using the homologous gene cloning method, and treated respectively with Streptomyces 769 fermentation broth and Alternanthera philoxeroides germ suspension for one hundred days Grass plants were used to detect the differences in chitinase gene expression levels by semi-quantitative RT-PCR. The results showed that Streptomyces 769 fermentation broth and Alternanthera philoxeroides suspension could induce the expression of Z. chinense chitinase gene at 24 h and 6 h after inoculation. Zinnia vetiver germ preferentially induced the expression of chitinase in plants, while the induction of Streptomyces 769 lagged. When Streptomyces strain 769 was sprayed with the pathogen for 36 h, the peak of chitinase gene expression in Streptomyces 769 appeared at 36 h after spraying, which was 12 h delayed than that induced by spraying alone. Streptomyces hygroscopicus 769 can induce systemic induced resistance in plants and induce different signal transduction pathways with P. zinniae pathogen to alleviate the plant diseases caused by pathogen infection.