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[目的]为保护野生苍术资源,并为南苍术工厂化生产提供技术指导。[方法]以南苍术(Atractylodes lancea(Thunb.)DC)种子为材料得到无菌试管苗,应用正交试验法L(934)研究6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)、激动素(KT)及其配比对南苍术无菌苗增殖的影响;NAA浓度对南苍术苗生根的影响;以泥土、河沙、棉籽壳为材料,采用正交试验方法设计9种轻型基质配方,研究其对南苍术生长的影响。[结果]适合南苍术试管苗增殖的培养基为MS+6RR鄄BA2.0mg/L+NAA0.4mg/L+KT0.4mg/L;适合南苍术生根的培养基为1/2MS+NAA0.5mg/L;最适基质配比为泥土:河沙:棉籽壳(体积比)=3:3:2。[结论]筛选出了南苍术增殖培养,生根的最佳配方,基质的最佳配比,为苍术资源的实验室保存和种苗工厂化生产提供了技术依据。
[Objective] In order to protect the resources of wild herb, and provide technical guidance for the industrialized production of [Method] With sterile seedlings of Atractylodes lancea (Thunb.) DC as seed material, the effects of 6-benzyl adenine (6-BA), NAA ), Kinetin (KT) and their proportions on the proliferation of Atractylodes lancea (Atrazus) atractylodes rhizome; the effect of NAA concentration on rooting of Atractylodes Junssiifolia seedlings; using the method of orthogonal experiment to design nine kinds of soil Lightweight matrix formulations to study its impact on the growth of the South Atractylodes. [Result] The medium suitable for the proliferation of Atractylodes macrocephala plantlets was MS + 6RR-BA2.0mg / L + NAA0.4mg / L + KT0.4mg / L. The medium suitable for rooting of Atractylodes macrocephala was 1 / 2MS + NAA0.5mg / L; the optimum matrix ratio of soil: river sand: cotton seed hulls (volume ratio) = 3: 3: 2. [Conclusion] The optimal formula of rooting, the best ratio of matrix, the laboratory preservation and the factory seed production of Atractylodes macrocephala resources were screened out.