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采用荧光光谱技术研究不同溶解氧(DO)水平下二十埠河底泥上覆水中溶解性有机物(DOM)转化特性及类蛋白荧光强度与总氮浓度的关系。三维荧光光谱显示:上覆水中DOM主要由三种类蛋白物质(高激发波长类酪氨酸、低激发波长类酪氨酸、低激发波长类色氨酸)和两种类富里酸物质(紫外区类富里酸、可见区类富里酸物质)组成,类蛋白物质是上覆水中DOM的主要成分。经过曝气后类蛋白荧光强度均存在明显降低,其中低激发波长酪氨酸和低激发波长色氨酸相对于高激发波长酪氨酸更易被微生物降解。而类富里酸荧光强度则均呈现增强趋势,表明类富里酸物质属于难降解有机物。上覆水中DOM荧光指数介于1.65~1.8之间,表明上覆水体DOM既有陆源又有生物源但以生物源为主。荧光指数随DO增加而增大,说明随着DO增加微生物量及微生物活性逐渐增加,微生物代谢功能增强,使得上覆水中DOM的生物源成份加大。在较高的溶解氧水平下,即DO分别为2.5,3.5和5.5mg·L-1时,高激发波长类酪氨酸峰A的荧光强度与上覆水中总氮浓度有良好的相关性,相关系数(r2)分别为0.956,0.946,0.953,说明可以通过三维荧光技术监测高激发波长类酪氨酸峰A的荧光强度而快速分析上覆水中总氮浓度,为河道水体诊断、治理及修复提供快速有效的技术参考和理论支持。
Fluorescence spectroscopy was used to study the transformation characteristics of DO and the fluorescence intensity of protein-like proteins in the overlying water of the 20-Bu River sediments under different dissolved oxygen (DO) levels. Three-dimensional fluorescence spectra showed that the DOM in the overlying water mainly consisted of three kinds of protein substances (tyrosine with high excitation wavelength, tyrosine with low excitation wavelength, tryptophan with low excitation wavelength) and two kinds of fulvic acid species Fulvic acid, the visible region of the type of fulvic acid substances), protein-like substances is the main component of the overlying water DOM. After the aeration, the fluorescence intensity of the protein was significantly decreased, of which tyrosine with low excitation wavelength and tyrosine with low excitation wavelength were more susceptible to microbial degradation than tyrosine with high excitation wavelength. While the fluorescence intensity of the fulvic acid showed an increasing trend, indicating that the fulvic acid-like substance is a refractory organic substance. The DOM fluorescence index of the overlying water ranged from 1.65 to 1.8, indicating that the DOM of the overlying water was both a terrestrial and a biological source but mainly of biological origin. The fluorescence index increased with the increase of DO, indicating that with the increase of DO and the increase of microbial activity, the metabolic function of microorganisms increased and the biogenic components of DOM in the overlying water increased. At higher dissolved oxygen levels (DOs of 2.5, 3.5 and 5.5 mg · L -1, respectively), the fluorescence intensity of tyrosine peak A at high excitation wavelength had a good correlation with the total nitrogen concentration in overlying water, The correlation coefficients (r2) were 0.956,0.946,0.953, respectively, indicating that the fluorescence intensity of tyrosine peak A with high excitation wavelength can be monitored by three-dimensional fluorescence technique and the concentration of total nitrogen in overlying water can be quickly analyzed to diagnose, treat and repair river water body Provide fast and effective technical reference and theoretical support.