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目的了解东营地区临床分离鲍曼不动杆菌的耐药性以及耐药基因同源性。方法收集2011年1-12月东营地区三级综合医院各类临床标本中分离的251株鲍曼不动杆菌,用K-B法进行药敏试验,PCR扩增主要碳青霉烯酶基因,用ERIC-PCR法进行DNA同源性分析。结果 251株鲍曼不动杆菌对哌拉西林耐药率最高,为76.2%,其次为甲氧苄啶/磺胺甲恶唑72.1%、头孢噻肟70.1%;耐亚胺培南/美罗培南鲍曼不动杆菌23株。同时携带OXA-23gp、OXA-24gp、OXA-64gp基因20株,携带OXA-58gp、OXA-64gp和PER基因3株;同源性分析显示,A1型15株,A2型1株,B型3株,C型2株,D型2株。结论东营地区鲍曼不动杆菌耐药严重,尚未发现同一克隆株的流行播散。
Objective To understand the clinical isolates of Acinetobacter baumannii in Dongying and the homology of drug resistance genes. Methods 251 strains of Acinetobacter baumannii isolated from various clinical specimens of Grade III General Hospital of Dongying from January to December, 2011 were collected. Susceptibility test was performed by KB method. The major carbapenemase genes were amplified by PCR, -PCR method for DNA homology analysis. Results 251 strains of Acinetobacter baumannii showed the highest drug resistance rate to piperacillin, which was 76.2%, followed by 72.1% of trimethoprim / sulfamethoxazole and 70.1% of cefotaxime. Imipenem / Meropenem 23 strains of Acinetobacter baumannii. Twenty OXA-23gp, OXA-24gp and OXA-64gp genes were also carried along with carrying OXA-58gp, OXA-64gp and PER genes. Homology analysis showed that there were 15 strains of A1, 1 of type A2 and 3 of type B 2 strains of C type and 2 strains of D type. Conclusion Acinetobacter baumannii in Dongying area was seriously drug-resistant, and no epidemic spread of the same clone was found.