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为揭示川硬皮肿腿蜂寄生对寄主黄粉虫蛹的调控机制,本文通过自然寄生和毒液注射等方法研究了寄生过程中蜂毒液的作用.结果表明:对人工注射的蛹,寄主被麻痹的程度与毒液注入量呈正相关,恢复活动情况与毒液注入量呈负相关;当人工注射毒液浓度为0.01 VRE时,黄粉虫表现出可逆的轻微麻痹;当浓度提高到0.2 VRE时,黄粉虫蛹表现出不可逆的完全僵化.单独注射土壤菌液,导致蛹大量死亡并出现大范围的黑化现象,而注射毒液和土壤菌液混合物,蛹的黑化作用发生延缓,且死亡率显著下降.对毒液的抑菌测试表明,毒液对金黄葡萄球菌的抑菌作用显著高于大肠杆菌.在一定温度范围内,随着温度升高毒液麻痹活性显著降低,对大肠杆菌抑菌性显著升高,对金黄葡萄球菌抑菌性显著降低.表明川硬皮肿腿蜂毒液对寄主黄粉虫蛹具有麻痹、抑菌、抑制蜕皮和延缓黑化的作用.
In order to reveal the regulatory mechanism of parasitoids of Cryptomeria rupestris on the host Tenebrio pupae, we studied the effect of bee venom in the process of parasitism by means of natural parasitism and venom injection.The results showed that for the pupae injected manually, the host was paralyzed The level of venom injection was positively correlated with the amount of venous injection, and the recovery was negatively correlated with the volume of venous injection. Tenebrio molitor showed reversible slight paralysis when the venom concentration was 0.01 VRE. When the concentration increased to 0.2 VRE, Irreversible complete rigidification.Under injection of soil bacteria alone, resulting in a large number of pupa death and a wide range of blackening phenomenon, and the injection of liquid and soil mixture of bacteria, pupation melanization delay, and mortality decreased significantly. The bacteriostasis test showed that the venom had stronger antibacterial activity against Staphylococcus aureus than E. coli.In a certain temperature range, the venom paralytic activity decreased significantly with the increase of temperature, the bacteriostasis against E.coli was significantly increased, Staphylococcus aureus bacteriostatis significantly reduced, indicating that the hard layer of Scleroderma venom paralysis on the host Tenebrio pupae, antibacterial, inhibit molting and delay the role of blackening.