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本研究建立了一种先在体外培养形成白血病、肿瘤干细胞克隆后,再注入易感动物体内传代,发病后在体外连续培养建成白血病、肿瘤细胞系的方法。这种体内、外结合的肿瘤细胞系建系方法较好解决了体内细胞不完全适应体外生长的困难。实验还证明,在原代细胞培养过程中加入PHA脾细胞条件培养液可促进白血病、肿瘤干细胞的增殖,使~3H-TdR参入增加,从而有助于体外白血病、肿瘤细胞克隆的形成.经
In this study, a method was developed to culture leukemia and tumor stem cells first in vitro, then inject them into susceptible animals, and then to establish a continuous leukemic and tumor cell line in vitro after the onset of disease. The in vivo and in vitro combination of the tumor cell line method is better to solve the problem that the cells in vivo do not fully adapt to the growth in vitro. The experiment also proved that the addition of PHA spleen cell conditioned medium during the primary cell culture process can promote the proliferation of leukemia, cancer stem cells, and increase the incorporation of ~3H-TdR, thus contributing to the formation of leukemia and tumor cell clones in vitro.