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目的研究日本血蜱传播田鼠巴贝西虫的能力。方法用日本血蜱幼蜱、若蜱和成蜱叮咬田鼠巴贝西虫阳性BALB/c小鼠,收集饱血蜱,待孵化为下一个龄期或产卵后,利用巢氏PCR法检测幼蜱的感染情况;再用感染性成蜱叮咬阴性NOD/SCID小鼠,定期采集鼠血进行检测,以观察小鼠感染情况。结果幼蜱叮咬阳性小鼠发育为若蜱后,未检出田鼠巴贝西虫感染;若蜱叮咬阳性小鼠发育为成蜱后,8只雌性成蜱检出田鼠巴贝西虫感染,而雄性成蜱未检出田鼠巴贝西虫感染;成蜱叮咬阳性小鼠后,子代幼蜱未检出田鼠巴贝西虫感染。而12只阴性NOD/SCID小鼠被阳性成蜱叮咬后,有2只小鼠感染。结论日本血蜱仅在若蜱-成蜱阶段可经期传播田鼠巴贝西虫,幼蜱-若蜱阶段不能经期传播田鼠巴贝西虫,亦不能经卵传播田鼠巴贝西虫。
Objective To study the ability of B. japonicus to transmit the Babesia spp. Methods BALB / c mice were infected with Babesia japonicum-positive BALB / c mice by ticks and ticks. After hatching for the next instar or spawning, the nested PCR method was used to detect young Tick infection; then infective tick tick bites negative NOD / SCID mice, blood samples were collected regularly to detect mouse infection. Results There was no infection of Babesia spp. In the young mice after the bite-positive mice developed ticks. If the tick-bite-positive mice developed into ticks, the infection of Babesia spp. Was detected in 8 female ticks Male ticks were not detected in field mice Babesia infection; Tick tick bite positive mice, the offspring ticks did not detect voles Babesia insect infection. Twelve negative NOD / SCID mice were bitten by positive ticks and 2 were infected. Conclusion The blood smear of Babesia japonicus could be transmitted only by the tick-tick stage in the tick-tick stage. The tick-tick-tick could not transmit the Babesia japonica through the tick or menstrual cycle.