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目的研究丙酮酸乙酯(ethyl pyruvate,EP)对宫内感染致新生仔鼠脑损伤12-脂氧合酶表达和细胞凋亡的影响。方法 36只孕鼠随机分为LPS组、EP组、NS组,每组各12只。LPS组:于孕17天、18天孕鼠连续两天腹腔注射脂多糖380μg/kg;EP组:相同的方法制备孕鼠宫内感染模型,并于孕鼠腹腔注射LPS后立即给予腹腔注射EP 40mg/kg;NS组:给予等量生理盐水腹腔注射。孕鼠分娩后,取胎盘进行HE染色,以观察宫内感染情况。剔除早产鼠,随机选取各组仔鼠36只,分别于出生后1d、3d、7d进行检测,观察脑组织12-脂氧合酶(12-lipoxygenase,12-LOX)的表达和神经细胞凋亡情况。结果与NS组比较,LPS组孕鼠胎盘组织可见明显的炎性细胞浸润,血管充血等病理改变;与LPS组相比较,EP组仔鼠出生后1、3、7d脑组织12-LOX的表达量均低于LPS组(P<0.05)、神经细胞凋亡指数均也均低于LPS组(P<0.05)。结论 EP对宫内感染致脑损伤新生仔鼠的脑组织具有保护作用,可能与12-LOX表达水平和细胞凋亡降低相关。
Objective To investigate the effects of ethyl pyruvate (EP) on the expression of 12-lipoxygenase and the apoptosis of brain in neonatal rats with intrauterine infection. Methods Thirty-six pregnant rats were randomly divided into LPS group, EP group and NS group, 12 rats in each group. LPS group: Pregnant mice were intraperitoneally injected with lipopolysaccharide 380μg / kg on day 17 and day 18 for 18 days. EP group: The same method was used to prepare intrauterine infection model in pregnant mice, and intraperitoneal injection of EP 40mg / kg; NS group: given the same amount of saline intraperitoneal injection. Pregnant mice after delivery, take the placenta HE staining to observe intrauterine infection. Thirty-six preterm infants were randomly selected from each preterm group. The rats were sacrificed at 1 day, 3 days and 7 days after birth respectively. The expression of 12-lipoxygenase (12-LOX) and neuronal apoptosis Happening. Results Compared with the NS group, the placental tissue of the LPS group showed obvious inflammatory cell infiltration and vascular congestion and other pathological changes; compared with the LPS group, the expression of 12-LOX in the brain of the EP group at 1, 3 and 7 days after birth (P <0.05), and the apoptotic index of nerve cells in LPS group were also lower than those in LPS group (P <0.05). Conclusion EP has a protective effect on the brain tissue of newborn offspring induced by intrauterine infection, which may be related to the decrease of 12-LOX expression and apoptosis.