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用2个DNA探针pJEL101和pBSavrXa10对78个水稻白叶枯病菌系进行RFLP分型,以分析其群体结构和遗传多样性。分别鉴定出16种RFLP标记带的谱型。以彼此的带位相似率达85％为界,可分为6簇。参试菌系的群体遗传多样性为0.77(用pJEL101),和0.83(用pBSavrXa10)。RFLP谱型分析表明:我国多数病原型为杂合组群。白叶枯病菌系的分子表现型的变异,远远大于致病型,两个探针都能有效分析我国菌系的群体结构,分子技术可用以进一步完善我国水稻与白叶枯病菌系之间的鉴别反应系统和新小种的侦察。
Two strains of rice bacterial blight were RFLP genotyped with two DNA probes pJEL101 and pBSavrXa10 to analyze their population structure and genetic diversity. Sixteen RFLP marker bands were identified. To each other with a bit similar rate of 85% for the community, can be divided into 6 clusters. The population genetic diversity of the tested strains was 0.77 (with pJEL101), and 0.83 (with pBSavrXa10). RFLP pattern analysis showed that most of the pathogenicity in China was heterozygous. The molecular phenotypic variation of bacterial blight is far greater than that of pathogenicity. Both probes can effectively analyze the population structure of Chinese bacterial strains, and molecular techniques can be used to further improve the relationship between the rice bacterial blight bacterial strains in China Identification of the reaction system and the detection of new race.