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以昆明小鼠为实验模型,连续进行GANRA类药物灌胃3d后,使用8Gy 100kVp的X射线进行辐照处理,然后对血象、脏器指数、小鼠存活率、肝脏中丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性等参数进行了统计分析。结果表明:(1)药物灌胃对小鼠存活率没有影响。辐照后,小鼠存活率显著下降,存活率下降至50%所需时间分别为:照射对照组20d、DMSO组9d、1#药物组29d、5#药物组24d;(2)药物灌胃对小鼠脏器指数没有明显影响,辐照后的对照组和DMSO组小鼠的肝脏指数、脾脏指数升高,药物组的脏器指数低于照射对照组和DMSO组;(3)辐照导致各实验组的MDA水平升高、SOD活性下降,但是5#药物的SOD活性高于DMSO组、MDA水平低于DMSO组;(4)药物灌胃对小鼠血象没有产生明显毒性效应;辐照后各实验组小鼠的白细胞等都显著下降。从上述实验结果可以看出,GANRA类1#和5#药物具有抗辐射作用,其机制可能与GANRA类药物的自由基清除能力有关。
Using Kunming mice as experimental model, GANRA was continuously administered for 3 days after gavage, and 8 Gy of 100 kVp X-ray was used for irradiation treatment. Then blood, organ index, survival rate of mice, malondialdehyde (MDA) Content and superoxide dismutase (SOD) activity parameters such as statistical analysis. The results showed that: (1) drug gavage had no effect on the survival rate of mice. After irradiation, the survival rate of mice was significantly decreased, the survival time required to reduce the time to 50% were: irradiation control group 20d, DMSO group 9d, 1 # drug group 29d, 5 # drug group 24d; (2) There was no significant effect on the organ index of mice. The index of liver and spleen of mice in DMSO group and DMSO group were higher than those in DMSO group and DMSO group. (3) Which led to the increase of MDA level and the decrease of SOD activity in each experimental group. However, SOD activity of 5 # drug was higher than DMSO group and MDA level was lower than that of DMSO group. (4) According to the experimental mice after irradiation, the white blood cells and the like were significantly decreased. From the above experimental results, it can be seen that the GANRA type 1 # and 5 # drugs have the anti-radiation effect, and the mechanism may be related to the free radical scavenging ability of the GANRA type drugs.