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目的： 观察链脲佐菌素（ＳＴＺ）糖尿病大鼠补充硒（Ｓｅ）和／或维生素Ｅ（ＶＥ）后红细胞内游离钙浓度［Ｃａ２＋ ］ｉ的变化。方法： 用ＳＴＺ制成糖尿病（ＤＭ）大鼠，分为四组：（１）ＤＭ 对照组；（２）Ｓｅ 治疗组；（３）ＶＥ治疗组；（４）Ｓｅ 和ＶＥ治疗组；另有正常对照组，每天以同体积的生理盐水灌胃。实验期为３０ 天，分析血Ｓｅ、ＶＥ及红细胞［Ｃａ２＋ ］ｉ。 结果： 补充Ｓｅ 和／或ＶＥ后，红细胞［Ｃａ２＋ ］ｉ显著下降，与ＤＭ 对照组比较，差异有显著意义（Ｐ＜ ０．０５，Ｐ＜ ０．０１），以联合用药组降幅较大。血硒水平联合用药组高于其他各组（Ｐ＜ ０．０１），补Ｓｅ组高于补ＶＥ组和ＤＭ 对照组（Ｐ＜ ０．０１）；血清ＶＥ浓度联合用药组高于ＤＭ 对照组和补Ｓｅ 组（Ｐ＜ ０．０１，Ｐ＜ ０．０５），而补ＶＥ组血清ＶＥ浓度已恢复至正常水平。 结论： Ｓｅ和ＶＥ对ＤＭ 大鼠红细胞［Ｃａ２＋ ］ｉ异常升高有明显的抑制作用。
Objective: To observe the change of intracellular free calcium concentration [Ca2 +] i in streptozotocin (STZ) diabetic rats after selenium (Se) and / or vitamin E (VE) supplementation. Methods: Diabetic rats were divided into four groups: (1) DM control group, (2) Se treatment group, (3) VE treatment group, (4) Se and VE treatment group and Normal control group, daily with the same volume of normal saline gavage. The experimental period was 30 days, blood Se, VE and red blood cells [Ca2 +] i were analyzed. Results: After adding Se and / or VE, [Ca2 +] i of erythrocytes decreased significantly compared with DM control group (P <0.05, P <0.01). The blood selenium level was higher in the combination group than in other groups (P <0.01), the level of selenium in the Se group was higher than that in the VE group and DM control group (P <0.01) And Se group (P <0.01, P <0.05), while the serum VE concentration in the VE group was restored to the normal level. Conclusion: Se and VE significantly inhibit the abnormal increase of [Ca2 +] i in erythrocytes of DM rats.